| Title | Structural Analysis of the fcbABC Gene Cluster Responsible for Hydrolytic Dechlorination of 4-Chlorobenzoate from pJS1 Plasmid of Comamonas sp. P08 |
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| Author | Jeong-Soon Lee 1 , Kyoung Lee 2 , Jong-Ok Ka 3 , Jong-Chan Chae 4 , and Chi-Kyung Kim 1, * |
| Address | 1 Department of Microbiology and Biotechnology, Chungbuk National University, Cheongju 361-763, Korea; 2 Department of Microbiology, Changwon National University, Changwon 641-773, Korea; 3 Department of Agricultural Biology, Seoul National University, Suwon 441-744, Korea; 4 Biotechnology Center for Agriculture and Environment, Rutgers University, New Brunswick, New Jersey 08901, USA (Received February 14, 2003 / Accepted March 13, 2003) |
| Bibliography | Journal of Microbiology, 41(2),89-94, 2003, |
| DOI | |
| Key Words | fcb genes, pJS1 plasmid, hydrolytic dechlorination, 4-chlorobenzoate, Comamonas sp. P08 |
| Abstract | Bacterial strain No. P08 isolated from wastewater at the Cheongju industrial complex was found to be capable of degrading 4-chlorobenzoate under aerobic condition. P08 was identified as Comamonas sp. from its cellular fatty acid composition and 16S rDNA sequence. The fcb genes, responsible for the hydrolytic dechlorination of 4-chlorobenzoate, were cloned from the plasmid pJS1 of Comamonas sp. P08. The fcb gene cluster of comamonas sp. P08 was organized in the order fcbB-fcbA-fcbT1-fcbT2-fcbT3-fcbC. This organization of the fcb genes was very similar to that of the fcb genes carried on the chromosomal DNA of Pseudomonas sp. DJ-12. However, it differed from the fcbA-fcbB-fcbC ordering of Arthrobacter sp. SU. The nucleotide sequences of the fcbABC genes of strain P08 showed 98% and 53% identities to those of Pseudomonas sp. DJ-12 and Arthrobacter sp. SU, respectively. This suggests that the fcb genes might have been derived from Pseudomonas sp. DJ-12 to form plasmid pJS1 in Comamonas sp. P08, or that the fcb genes in strain DJ-12 were transposed from Comamonas sp. P08 plasmid. |
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