| Title | Isolation and Characterization of Bud6p, an Actin Interacting Protein, from Yarrowia lipolytica |
|---|---|
| Author | Yunkyoung Song, Seon Ah Cheon, So-Yeon Lee, Ji-Sook Hwang, and Jeong-Yoon Kim * |
| Address | Department of Microbiology and Institute of Biotechnology, Chungnam National University, Daejeon 305-764, Korea (Received February 25, 2003 / Accepted May 26, 2003) |
| Bibliography | Journal of Microbiology, 41(2),121-128, 2003, |
| DOI | |
| Key Words | Yarrowia lipolytica, BUD6, yeast, hyphae, cell polarity |
| Abstract | The identification of genes involved in true hypha formation is important in the study of mechanisms underlying the morphogenetic switch in yeast. We isolated a gene responsible for the morphogenetic switch in Yarrowia lipolytica, which forms true hyphae in response to serum or N-acetylglucosamine. The isolated gene, encoding 847 amino acids, had sequence identities of 27% and 25% with the Bud6 (Aip3) proteins of Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. Disruption of this gene, designated YlBUD6, in haploid and diploid strains significantly reduced the ability of Y. lipolytica to switch from the yeast form to the hyphal form in hypha-inducing media. It was also found that YlBud6 mutants were rounder than the wild type when grown in the yeast form. These results indicate that the YlBud6 protein is necessary for hyphal growth and cell polarity in both haploid and diploid Y. lipolytica cells. |
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