| Title | Culture and Identification of Bacteria from Marine Biofilms |
|---|---|
| Author | Yoo Kyung Lee, Kae-Kyung Kwon, Kyeung Hee Cho, Hyo Won Kim, Jae Hyun Park, and Hong Kum Lee * |
| Address | Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan, P.O. Box 29, 425-600, Korea (Received May 17, 2003 / Accepted June 19, 2003) |
| Bibliography | Journal of Microbiology, 41(3),183-188, 2003, |
| DOI | |
| Key Words | biofilm, [alpha]-Proteobacteria, [gamma]-Proteobacteria, CFB group bacteria, Gram-positive bacteria |
| Abstract | We isolated and cultured bacteria that inhabited marine biofilms, and identified them by phylogenetic analysis using 16S rDNA sequences. In the marine environment, biofilms cover most subtidal and intertidal solid surfaces such as rocks, ships, loops, marine animals, and algae. The bacteria in most biofilms are embedded in extracellular polymeric substances that comprise mainly of exopolysaccharides. The exopolysaccharides are excreted from multiple bacterial species; therefore, biofilms are a good source for screening exopolysaccharide-producing bacteria. Thirty-one strains were cultured, and a total of 17 unique strains were identified. Phylogenetic analysis using 16S rDNA sequences indicated that the 17 strains belonged to [alpha]-Proteobacteria (Ochrobactrum anthropi, Paracoccus carotinifaciens); [gamma]-Proteobacteria (Pseudoalteromonas agarovorans, P. piscicida, Pseudomonas aeruginosa, Shewanella baltica, Vibrio parahaemolyticus, V. pomeroyi); CFB group bacteria (Cytophaga latercula, Tenacibaculum mesophilum); high GC, Gram-positive bacteria (Arthrobacter nicotianae, Brevibacterium casei, B. epidermidis, Tsukamurella inchonensis); and low GC, Gram-positive bacteria (Bacillus macroides, Staphylococcus haemolyticus, S. warneri). |
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