| Title | Rapid Identification of Vibrio vulnificus in Seawater by Real-Time Quantitative TaqMan PCR |
|---|---|
| Author | Hye-Young Wang1 and Geon-Hyoung Lee 2 , * |
| Address | 1 Mol-BioNet Research Institute, Scientec Lab Center Co., Ltd. College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeon-buk 561-756, Korea.; 2 Dept. of Biology, College of Natural Sciences, Kunsan National University, Gunsan, Jeon-buk 573-701, Korea |
| Bibliography | Journal of Microbiology, 41(4),320-326, 2003, |
| DOI | |
| Key Words | Vibrio vulnificus, conventional PCR, real-time quantitative TaqMan PCR, Biolog^TM system |
| Abstract | In order to identify Vibrio vulnificus in the Yellow Sea near Gunsan, Korea during the early and late summers, the efficiency of the real-time quantitative TaqMan PCR was compared to the efficiency of the conventional PCR and Biolog identification system^TM. Primers and a probe were designed from the hemolysin/cytolysin gene sequence of V. vulnificus strains. The number of positive detections by real-time quantitative TaqMan PCR, conventional PCR, and the Biolog identification system from seawater were 53 (36.8%), 36 (25%), and 10 strains (6.9%), respectively, among 144 samples collected from Yellow Sea near Gunsan, Korea. Thus, the detection method of the real-time quantitative TaqMan PCR assay was more effective in terms of accuracy than that of the conventional PCR and Biolog system. Therefore, our results showed that the real-time TaqMan probe and the primer set developed in this study can be applied successfully as a rapid screening tool for the detection of V. vulnificus. |
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