| Title | Schizosaccharomyces pombe rsm1 Genetically Interacts with spmex67, Which Is Involved in mRNA Export |
|---|---|
| Author | Jin Ho Yoon |
| Address | Division of Biology, Co11ege of Natural Sciences, Kangnung National University, Gangneung 210-702, Korea |
| Bibliography | Journal of Microbiology, 42(1),32-36, 2004, |
| DOI | |
| Key Words | rsm1, mex67, synthetic lethality, mRNA export, Schizosaccharomyces pombe |
| Abstract | We have previously isolated three synthetic lethal mutants from Schizosaccharomyces pombe in order to identify mutations in the genes that are functionally linked to spmex67 with respect to mRNA export. A novel rsm1 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex1. The rsm1 gene contains no introns and encodes a 296 amino-acid-long protein with the RING finger domain, a C3HC4 in the N-terminal half. The [delta]rsm1 null mutant is viable, but it showed a slight poly(A)^+ RNA accumulation in the nucleus. Also, the combination of [delta]rsm1 and [delta]spmex67 mutations confers synthetic lethality that is accompanied by the severe poly(A)^+ RNA export defect. These results suggest that rsm1 is involved in mRNA export from the nucleus. |
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