Title The Influence of NaCl and Carbonylcyanide-m-Chlorophenylhydrazone on the Production of Extracellular Proteases in a Marine Vibrio Strain
Author Young Jae Kim
Address Department of Microbiology, Changwon National University, Sarim-dong, Changwon, Kyungnam 641-773, Republic of Korea
Bibliography Journal of Microbiology, 42(2),156-159, 2004,
DOI
Key Words a marine bacterium, Vibrio sp. strain 60, extracellular protease producton, NaCl, CCCP
Abstract In general, the salinity of the ocean is close to 3.5% and marine vibrios possess the respiratory chainlinked Na+ pump. The influence of sodium chloride and the proton conductor carbonylcyanide m-chlorophenylhydrazone (CCCP) on the production of extracellular proteases in a marine Vibrio strain was examined. At the concentration of 0.5 M, sodium chloride minimally inhibited the activity of extracellular proteases by approximately 16%, whereas at the same concentration, the producton of extracellular proteases was severely inhibited. On the other hand, the production of extracellular proteases was completely inhibited by the addition of 2 μM CCCP at pH 8.5, where the respiratory chain-linked Na^+ pump functions.
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