| Title | Green Fluorescent Protein as a Marker for Monitoring a Pentachlorophenol Degrader Sphingomonas chlorophenolica ATCC39723 |
|---|---|
| Author | Eun-Taex Oh 2 , Jae-Seong So 2, Byung-Hyuk Kim 1 , Jong-Sul Kim 3 , and Sung-Cheol Koh 1 , * |
| Address | 1 Division of Civil and Environmental Systems Engineering, Korea Maritime University, Busan 606-791, Korea ; 2 Department of Biological Engineering, Center for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea; 3 Division of Life Science, University of Ulsan, Ulsan 680-749, Korea |
| Bibliography | Journal of Microbiology, 42(3),243-247, 2004, |
| DOI | |
| Key Words | gfp, microcosm, monitoring, pentachlorophenol (PCP), Sphingomonas chlorophenolica |
| Abstract | Sphingomonas chlorophenolica ATCC39723 was successfully labeled with the gfp (green fluorescent protein) gene inserted into the pcpB gene by homologous recombination. As the gfp recombinant was easily distinguished from other indigenous organisms, the population of gfp recombinant was monitored after being released into the soil microcosms. Their population density dropped from 108 to 106 (cfu/ml) in the non-sterilized soil microcosms during the first 6 days. Moreover, the gfp recombinant was not detected even at lower dilution rates after a certain time period. The recombinant, however, survived for at least 28 days in the sterilized soil microcosms. Although the gfp recombinant did not degrade pentachlorophenol (PCP), this experiment showed the possibility of using gfp as a monitoring reporter system for S. chlorophenolica ATCC39723 and potentially other species of Sphingomonas. |
| Download PDF | p.243-247.pdf |