| Title | Isolation and Characterization of a-Glucosidase Inhibitor from the Fungus Ganoderma lucidum |
|---|---|
| Author | Shin-Duk Kim * and Hong Joon Nho |
| Address | Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea |
| Bibliography | Journal of Microbiology, 42(3),223-227, 2004, |
| DOI | |
| Key Words | [alpha]-glucosidase inhibitor, Ganoderma lucidum, reversible, competitive inhibitor |
| Abstract | An [alpha]-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of [alpha]-glucosidase. It showed very potent inhibitory activity against [alpha]-glucosidase with an IC_50 value of 4.6 mg/ml, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Lineweaver-Burk plot indicated that the SKG-3 inhibition of [alpha]-glucosidase was competitive. |
| Download PDF | p.223-227.pdf |