| Title | Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants |
|---|---|
| Author | Hyo Shim Han 1, Young Jin Koh 2, Jae-Seoun Hur 3, and Jae Sung Jung 1,* |
| Address | 1Department of Biology, Sunchon National University, Sunchon 540-742, Republic of Korea, 2Department of Applied Biology, Sunchon National University, Sunchon 540-742, Republic of Korea, 3Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea |
| Bibliography | Journal of Microbiology, 42(4),365-368, 2004, |
| DOI | |
| Key Words | Pseudomonas syringae pv. syringae, kiwifruit, strA-strB, Tn5393 |
| Abstract | The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA→CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg. |
| Download PDF | p.365-3680.pdf |