| Title | Characteristics of HIV-Tat Protein Transduction Domain |
|---|---|
| Author | Jong-Sub Yoon1, Yong-Tae Jung 4, Seong-Karp Hong3, Sun-Hwa Kim1, Min-Chul Shin1, Dong-Gun Lee 2, Wan-Shik Shin 2, Woo-Sung Min 2, and Soon-Young Paik 1,* |
| Address | 1Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea, 2Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea, 3Catholic Hemopoietic Stem Cell Transplantation Center, The Catholic University of Korea, Seoul, 137-701, Republic of Korea, 4Department of Microbiology, College of Advanced Science, Dankook University, Seoul 140-714, Republic of Korea |
| Bibliography | Journal of Microbiology, 42(4),328-335, 2004, |
| DOI | |
| Key Words | HIV-1-Tat, protein trasduction domain, biodistribution |
| Abstract | The human immunodeficiency virus type 1 (HIV-1) Tat protein transduction domain (PTD), which contains rich arginine and lysine residues, is responsible for the highly efficient transduction of protein through the plasma membrane. In addition, it can be secreted from infected cells and has the ability to enter neighboring cells. When the PTD of Tat is fused to proteins and exogenously added to cells, the fusion protein can cross plasma membranes. Recent reports indicate that the endogenously expressed Tat fusion protein can demonstrate biodistribution of several proteins. However, intercellular transport and protein transduction have not been observed in some studies. Therefore, this study examined the intercellular transport and protein transduction of the Tat protein. The results showed no evidence of intercellular transport (biodistribution) in a cell culture. Instead, the Tat fusion peptides were found to have a significant effect on the transduction and intercellular localization properties. This suggests that the HIV-1 PTD passes through the plasma membrane in one direction. |
| Download PDF | p.328-335.pdf |