| Title | Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17 |
|---|---|
| Author | Dockyu Kim1,5, Jong-Chan Chae2, Gerben J. Zylstra2, Ho-Yong Sohn3, Gi-Seok Kwon4, and Eungbin Kim1,* |
| Address | 1Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea, 2Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA 3Department of Food and Nutrition, Andong National University, Andong 760-749, Republic of Korea, 4The School of Bioresource Science, Andong National University, Andong 760-749, Republic of Korea, 5Microbial Resources Bank, Microbial Genomics and Applications Center Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(1),49-53, 2005, |
| DOI | |
| Key Words | Rhodococcus, two-component signal transduction proteins, alkylbenzenes |
| Abstract | Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17. |
| Download PDF | p[1].49-53.pdf |