| Title | The Schizosaccharomyces pombe Gene Encoding [gamma]-Glutamyl Transpeptidase I Is Regulated by Non-fermentable Carbon Sources and Nitrogen Starvation |
|---|---|
| Author | Hong-Gyum Kim1, Hey-Jung Park1, Hyun-Jung Kang1, Hye-Won Lim1, Kyunghoon Kim1, Eun-Hee Park2, Kisup Ahn3, and Chang-Jin Lim1,* |
| Address | 1Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea, 3Department of Health and Environment, Baekseok College, Cheonan 330-705, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(1),44-48, 2005, |
| DOI | |
| Key Words | Schizosaccharomyces pombe, [gamma]-glutamyl transpeptidase, non-fermentable carbon sources, regulation, GGTI-lacZ fusion gene, Pap1, ethanol, acetate, nitrogen starvation |
| Abstract | In our previous study, the first structural gene (GGTI) encoding g-glutamyl transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of [beta]-galactosidase from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation. |
| Download PDF | p[1].44-48.pdf |