| Title | Isolation, Characterization, and Investigation of Surface and Hemolytic Activities of a Lipopeptide Biosurfactant Produced by Bacillus subtilis ATCC 6633 |
|---|---|
| Author | Gholamreza Dehghan-Noudeh1,*, Mohammadreza Housaindokht2, and Bibi Sedigeh Fazly Bazzaz3 |
| Address | 1Department of Pharmaceutics, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran, 2Department of Chemistry, School of Sciences, Ferdowsi University, Mashhad, Iran, 3Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran |
| Bibliography | Journal of Microbiology, 43(3),272-276, 2005, |
| DOI | |
| Key Words | Bacillus subtilis, biosurfactant, critical micelle concentration, hemolytic activity, surface tension |
| Abstract | Bacillus subtilis ATCC 6633 was grown in BHIB medium supplemented with Mn^2^+ for 96 h at 37^oC in a shaker incubator. After removing the microbial biomass, a lipopeptide biosurfactant was extracted from the supernatant. Its structure was established by chemical and spectroscopy methods. The structure was confirmed by physical properties, such as Hydrophile-Lipophile Balance (HLB), surface activity and erythrocyte hemolytic capacity. The critical micelle concentration (cmc) and erythrocyte hemolytic capacity of the biosurfactant were compared to those of surfactants such as SDS, BC (benzalkonium chloride), TTAB (tetradecyltrimethylammonium bromide) and HTAB (hexadecyltrimethylammonium bromide). The maximum hemolytic effect for all surfactants mentioned was observed at concentrations above cmc. The maximum hemolytic effect of synthetic surfactants was more than that of the biosurfactant produced by B. subtilis ATCC 6633. Therefore, biosurfactant would be considered a suitable surface-active agent due to low toxicity to the membrane. |
| Download PDF | p.272-276.pdf |