| Title | Prevalence of Putative Periodontopathogens in Subgingival Dental Plaques from Gingivitis Lesions in Korean Orthodontic Patients |
|---|---|
| Author | Seung Mi Lee1, So Young Yoo2, Hwa-Sook Kim2, Kwang-Won Kim1,3, Young-Joo Yoon1, Sung-Hoon Lim1,3, Hee-Young Shin4, and Joong-Ki Kook2,3,* |
| Address | 1Department of Orthodontics, College of Dentistry, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Republic of Korea, 2Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Republic of Korea, 3Oral Biology Research Institute, College of Dentistry, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Republic of Korea, 4Department of Preventive Medicine, Chonnam National University Medical School 5 Hak-dong, Dong-gu, Gwangju 501-746, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(3),260-265, 2005, |
| DOI | |
| Key Words | gingivitis, periodontopathogens, orthodontic patients, PCR, 16S rDNA |
| Abstract | The objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. Thirty-six individuals participated in this study. Ninteen of these subjects did not wear any orthodontic appliances, and these subjects comprised the control group. The other 17 individuals had been wearing fixed orthodontic appliances for at least 3 months each. After a periodontal examination, we collected subgingival plaque samples from the gingivitis lesions of each patient. Using PCR based on 16S rDNA, we detected the presence of 6 putative periodontopathogenic species, Treponema denticola, Porphyromonas gingivalis, Tannerella forsythia (formerly Bacteroides forsythus), Prevotella nigrescens, Prevotella intermedia, and Actinobacillus actinomycetemcomitans. With regard to the presence of individual periodontopathogens, we found that T. forsythia, T. denticola, and P. nigrescens were significantly more common in the samples obtained from the orthodontic patients than in the samples obtained from the non-orthodontic patient controls. Our results indicate that the local changes associated with the wearing of fixed orthodontic appliances may affect the prevalence of periodontopathogens in subgingival dental plaques. |
| Download PDF | p.260-265.pdf |