| Title | Cytochrome c_550 is Related to Initiation of Sporulation in Bacillus subtilis |
|---|---|
| Author | Inji Shin, Han-Bong Ryu, Hyung-Soon Yim*, and Sa-Ouk Kang* |
| Address | Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(3),244-250, 2005, |
| DOI | |
| Key Words | B. subtilis, cytochrome c_550, cccA, spo0A, sporulation |
| Abstract | The effect of cytochrome c_550 encoded by cccA in Bacillus subtilis during the event of sporulation was investigated. The sporulation of cccA-overexpressing mutant was significantly accelerated, while disruptant strain showed delayed sporulation in spite of the same growth rate. Activity of sporulation stage-0-specific enzyme, extracellular [alpha]-amylase of mutant strains was similar to that of the control strain, but cccA-overexpressing mutant exhibited higher activity of stage-II-specific alkaline phosphatase and stage-III-specific glucose dehydrogenase when compared to deletion mutant and control strain. Northern blot analysis also revealed that cccA-overexpressing mutant showed high level of spo0A transcripts, while the disruptant rarely expressed spo0A. These results suggested that although cytochrome c_550 is dispensable for growth and sporulation, expression of cccA may play an important role for initiation of sporulation through regulation of spo0A expression. |
| Download PDF | p.244-250.pdf |