| Title | Optimization of Bacteriocin ST311LD Production by Enterococcus faecium ST311LD, Isolated from Spoiled Black Olives |
|---|---|
| Author | Svetoslav D. Todorov and Leon M.T. Dicks* |
| Address | Department of Microbiology, Stellenbosch University, 7600 Stellenbosch, South Africa |
| Bibliography | Journal of Microbiology, 43(4),370-374, 2005, |
| DOI | |
| Key Words | Enterococcus faecium, bacteriocin ST311LD, black olives |
| Abstract | Bacteriocin ST311LD is approximately 2.3 kDa in size. Low levels of bacteriocin activity were recorded in BHI and M17 broth (800 AU/ml) and in 10% (w/v) soy milk (3,200 AU/ml). No bacteriocin production was recorded in 10% (w/v) molasses, despite good growth. Optimal levels (12,800 AU/ml) were detected in MRS broth which had been supplemented with tryptone (20.0 g/l), saccharose (5.0 or 10.0 g/l) or vitamin C (1 ppm). Increased potassium levels did not result in higher levels of activity, and glycerol (1.0 g/l) inhibited the production of bacteriocin ST311LD. |
| Download PDF | p.370-374.pdf |