| Title | Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T |
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| Author | Hwa-Sook Kim1, Soo Keun Song1, So Young Yoo1, Dong Chun Jin2, Hwan Seon Shin1, Chae Kwang Lim1, Myung-Soo Kim2, Jin-Soo Kim3, Son-Jin Choe4, and Joong-Ki Kook1,* |
| Address | 1Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, 2Department of Dental Pharmacology, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, 3Department of Oral and Maxillofacial Radiology, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, 4Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(4),331-336, 2005, |
| DOI | |
| Key Words | DNA probe Fu12, identification, Fusobacterium nucleatum ATCC 25586^T, strain-specific PCR primers |
| Abstract | The objective of this study was to assess the strain-specificity of a DNA probe, Fu12, for Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T (F. nucleatum ATCC 25586^T), and to develop sets of strain-specific polymerase chain reaction (PCR) primers. Strain-specificity was tested against 16 strains of F. nucleatum and 3 strains of distinct Fusobacterium species. Southern blot hybridization revealed that the Fu12 reacted exclusively with the HindIII-digested genomic DNA of F. nucleatum ATCC 25586^T. The results of PCR revealed that three pairs of PCR primers, based on the nucleotide sequence of Fu12, generated the strain-specific amplicons from F. nucleatum ATCC 25586^T. These results suggest that the DNA probe Fu12 and the three pairs of PCR primers could be useful in the identification of F. nucleatum ATCC 25586^T, especially with regard to the determination of the authenticity of the strain. |
| Download PDF | p.331-336.pdf |