| Title | Diversity of Denitrifying Bacteria Isolated from Daejeon Sewage Treatment Plant |
|---|---|
| Author | Young-Woon Lim1, Soon-Ae Lee2, Seung Bum Kim3, Hae-Young Yong2, Seon-Hee Yeon2, Yong-Keun Park4, Dong-Woo Jeong2, and Jin-Sook Park2,* |
| Address | 1Department of Wood Science, University of British Columbia, 2900-2424 Main Mall, Vancouver, B.C. V6T 1Z4, Canada, 2Department of Biotechnology, Hannam University, 133 Ojung-dong, Daeduk-gu, Daejeon 306-791, Republic of Korea, 3Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yusong-gu, Daejeon 305-764, Republic of Korea, 4Laboratory of Microbial Genetics, School of Life Science and Biotechnology, Korea University, 5 Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea |
| Bibliography | Journal of Microbiology, 43(5),383-390, 2005, |
| DOI | |
| Key Words | ARDRA, denitrifying bacteria, sewage treatment plant |
| Abstract | The diversity of the denitrifying bacterial populations in Daejeon Sewage Treatment Plant was examined using a culture-dependent approach. Of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. According to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. This grouping was supported by an unweighted pair group method, using an arithmetic mean (UPGMA) analysis with fatty acid profiles. Restriction pattern analysis of 16S rDNA with four endonucleases (AluI, BstUI, MspI and RsaI) again revealed seven distinct groups, consistent with those defined from the morphological and biochemical properties and fatty acid profiles. Through the phylogenetic analysis using the 16S rDNA partial sequences, the main denitrifying microbial populations were found to be members of the phylum, Proteobacteria; in particular, classes Gammaproteobacteria (Aeromonas, Klebsiella and Enterobacter) and Betaproteobacteria (Acidovorax, Burkholderia and Comamonas), with Firmicutes, represented by Bacillus, also comprised a major group. |
| Download PDF | 383-390.pdf |