| Title | Strain Improvement of Candida tropicalis for the Production of Xylitol:Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5 |
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| Author | Ravella Sreenivas Rao1,3, Cherukuri Pavana Jyothi11, Reddy Shetty Prakasham2, Chaganti Subba Rao2, Ponnupalli Nageshwara Sarma2, and Linga Venkateswar Rao1,* |
| Address | 1Department of Microbiology, Omania University, Hyderabad - 500 007, India, 2Biochemical and Environmental Engineering Centre, Indian Institute of Chemical Technology, Hyderabad - 500 007, India , 3W113, Centre for Cellular and Molecular Biology (CCMB), Uppal Road, Hyderabad - 500 007, India |
| Bibliography | Journal of Microbiology, 44(1),113-120, 2006, |
| DOI | |
| Key Words | Candida tropicalis, biochemical and physiological characterization, MNNG, mutagenesis,strain improvement, UV, xylitol, wild, mutant, yeast |
| Abstract | Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamydospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5. |
| Download PDF | 17 JM2005-119.pdf |