| Title | Morphological, Phylogenetic and Biological Characteristics of Ectropis obliqua Single-Nucleocapsid Nucleopolyhedrovirus |
|---|---|
| Author | Xiu-cui Ma1, Hai-Jun Xu1, Mei-Jun Tang2, Qiang Xiao2, Jian Hong1, and Chuan-Xi Zhang1,* |
| Address | 1Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang 310029, China, 2Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, Zhejiang 310000, China |
| Bibliography | Journal of Microbiology, 44(1),77-82, 2006, |
| DOI | |
| Key Words | Ectropis obliqua, single-nucleocapsid nucleopolyhedrovirus, polyhedrin, phylogeny, bioassay |
| Abstract | The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to 1.7 μm in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rr1, polyhedrin, orf1629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90) were conducted to test the susceptibility of E. obliqua larvae to the virus. |
| Download PDF | 12 JM2005-145.pdf |