| Title | Application of Bovine Viral Diarrhoea Virus as an Internal Control in Nucleic Acid Amplification Tests for Hepatitis C Virus RNA in Plasma-Derived Products |
|---|---|
| Author | Si Hyung Yoo, Seung Hee Hong, Sa Rah Jung, Su Jin Park, Nam Kyung Lee, Soon Nam Kim, Sang Mo Kang1, Hong Ki Min, Sue Nie Park, and Seung Hwa Hong* |
| Address | Biologics Headquarters, Korea Food and Drug Administration, 231 Jinheungno Eunpyeong-Gu, Seoul 122-704, Republic of Korea, 1Department of Microbial Engineering, KonKuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea |
| Bibliography | Journal of Microbiology, 44(1),72-76, 2006, |
| DOI | |
| Key Words | hepatitis C virus, nucleic acid amplification test, plasma-derived products, internal control |
| Abstract | Plasma-derived products are produced from plasma via fractionation and chromatography techniques, but can also be produced by other methods. In the performance of nucleic acid amplification tests (NAT) with plasma-derived products, it is necessary to include an internal control for the monitoring of all procedures. In order to avoid false negative results, we confirmed the usefulness of the bovine viral diarrhoea virus (BVDV) for use as an internal control in the detection of hepatitis C virus (HCV) RNA in plasma-derived products. These products, which were spiked with BVDV, were extracted and then NAT was performed. Specificity and sensitivity were determined via the adjustment of primer concentrations and annealing temperatures. BVDV detection allows for validation in the extraction, reverse transcription, and amplification techniques used for HCV detection in plasma-derived products. |
| Download PDF | 11 JM2005-102.pdf |