Title Enhanced Production of Exopolysaccharides by Fed-batch Culture of Ganoderma resinaceum DG-6556
Author Hyun Mi Kim1, Soon-Young Paik2, Kyung Soo Ra3, Kwang Bon Koo4, Jong Won Yun1, and Jang Won Choi4,*
Address 1Department of Biotechnology, College of Engineering, Daegu University, Kyungsan, Kyungbuk 712-714, Republic of Korea, 2Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea, 33Department of Food and Nutrition, Daegu Technical College, Daegu 704-721, Republic of Korea, 4Department of Bioindustry, College of Life & Environment, Daegu University, Kyungbuk 712-714, Republic of Korea
Bibliography Journal of Microbiology, 44(2),233-242, 2006,
DOI
Key Words exopolysaccharides, fed-batch culture, mushrooms, submerged culture, Ganoderma
Abstract The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM MnCl2 at an initial pH 6.0 and temperature 31°C. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l) were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose medium. It may be worth attempting with other mushroom fermentation processes for enhanced production of mushroom polysaccharides, particularly those with industrial potential.
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