| Title | Enhanced Production of Exopolysaccharides by Fed-batch Culture of Ganoderma resinaceum DG-6556 |
|---|---|
| Author | Hyun Mi Kim1, Soon-Young Paik2, Kyung Soo Ra3, Kwang Bon Koo4, Jong Won Yun1, and Jang Won Choi4,* |
| Address | 1Department of Biotechnology, College of Engineering, Daegu University, Kyungsan, Kyungbuk 712-714, Republic of Korea, 2Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea, 33Department of Food and Nutrition, Daegu Technical College, Daegu 704-721, Republic of Korea, 4Department of Bioindustry, College of Life & Environment, Daegu University, Kyungbuk 712-714, Republic of Korea |
| Bibliography | Journal of Microbiology, 44(2),233-242, 2006, |
| DOI | |
| Key Words | exopolysaccharides, fed-batch culture, mushrooms, submerged culture, Ganoderma |
| Abstract | The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM MnCl2 at an initial pH 6.0 and temperature 31°C. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l) were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose medium. It may be worth attempting with other mushroom fermentation processes for enhanced production of mushroom polysaccharides, particularly those with industrial potential. |
| Download PDF | 14 JM2006-032.pdf |