| Title | Improved Production of Live Cells of Lactobacillus rhamnosus by Continuous Cultivation using Glucose-yeast Extract Medium |
|---|---|
| Author | Liew Siew Ling1, Rosfarizan Mohamad*1, Raha Abdul Rahim2, Ho Yin Wan1, and Arbakariya Bin Ariff2 |
| Address | 1Laboratory of Enzyme and Microbial Technology, Institute of Bioscience Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia, 2Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia |
| Bibliography | Journal of Microbiology, 44(4),p.439-446, 2006, |
| DOI | |
| Key Words | probiotic, Lactobacillus rhamnosus, continuous culture, lactic acid, modeling |
| Abstract | In this study, the growth kinetics of Lactobacillus rhamnosus and lactic acid production in continuous culture were assessed at a range of dilution rates (0.05 h?1 to 0.40 h?1) using a 2 L stirred tank fermenter with a working volume of 600 ml. Unstructured models, predicated on the Monod and Luedeking-Piret equations, were employed to simulate the growth of the bacterium, glucose consumption, and lactic acid production at different dilution rates in continuous cultures. The maximum specific growth rate of L. rhamnosus, ┢max, was estimated at 0.40 h?1, and the Monod cell growth saturation constant, Ks, at approximately 0.25 g/L. Maximum cell viability (1.3 ≠ 1010 CFU/ml) was achieved in the dilution rate range of D = 0.28 h?1 to 0.35 h?1. Both maximum viable cell yield and productivity were achieved at D = 0.35 h?1. The continuous cultivation of L. rhamnosus at D = 0.35 h?1 resulted in substantial improvements in cell productivity, of 267% (viable cell count) that achieved via batch cultivation. |
| Download PDF | 10-2006-008.pdf |