| Title | Nucleocapsid Amino Acids 211 to 254, in Particular, Tetrad Glutamines, are Essential for the Interaction Between the Nucleocapsid and Membrane Proteins of SARS-Associated Coronavirus |
|---|---|
| Author | Xiaonan Fang, Lin-Bai Ye*, Yijuan Zhang, Baozong Li, Shanshan Li, Lingbao Kong, Yuhua Wang, Hong Zheng, Wei Wang, and Zhenghui Wu |
| Address | State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, P.R. China |
| Bibliography | Journal of Microbiology, 44(5),577-580, 2006, |
| DOI | |
| Key Words | SARS-CoV, membrane protein, nucleocapsid protein, M-N interaction，GST pull-down assay |
| Abstract | GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted. |
| Download PDF | JM 44(5)-150.pdf |