| Title | Cloning and Sequence Analysis of a Glyceraldehyde-3-phosphate Dehydrogenase Gene from Ganoderma lucidum |
|---|---|
| Author | Xu Fei, Ming Wen Zhao*, and Yu Xiang Li |
| Address | College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 and Key Laboratory of Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, P.R. China |
| Bibliography | Journal of Microbiology, 44(5),515-522, 2006, |
| DOI | |
| Key Words | gyceraldehyde-3-phosphate dehydrogenase gene, Ganoderma lucidum, medicinal mushroom, strain improvement, promoter, transformation vector |
| Abstract | A cDNA library of Ganoderma lucidum has been constructed using a Zap Express cloning vector. A glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was isolated from this library by hybridization of the recombinant phage clones with a gpd-specific gene probe generated by PCR. By comparison of the cDNA and the genomic DNA sequences, it was found that the complete nucleotide sequence encodes a putative polypeptide chain of 338 amino acids interrupted by 6 introns. The predicted amino acid sequence of this gene shows a high degree of sequence similarity to the GPD proteins from yeast and filamentous fungi. The promoter region contains a CT-rich stretch, two CAAT boxes, and a consensus TATA box. The possibility of using the gpd promoter in the construction of new transformation vectors is discussed. |
| Download PDF | JM 44(5)-06.pdf |