| Title | Studies on Synonymous Codon and Amino Acid Usage Biases in the Broad-Host Range Bacteriophage KVP40 |
|---|---|
| Author | Keya Sau1, Sanjib Kumar Gupta2, Subrata Sau3, Subhas Chandra Mandal1, and Tapash Chandra Ghosh2* |
| Address | 1Department of Mathematics, Jadavpur University Calcutta-700 032, India, 2Bioinformatics Centre, Bose Institute, P1/12-CIT Scheme VII M, Calcutta 700 054, India, 3Department of Biochemistry, Bose Institute, P1/12-CIT Scheme VII M, Calcutta 700 054, India |
| Bibliography | Journal of Microbiology, 45(1),58-63, 2007, |
| DOI | |
| Key Words | relative synonymous codon usage (RSCU), amino acid usage, correspondence analysis, phage KVP40, GRAVY, mean molecular weight |
| Abstract | In this study, the relative synonymous codon and amino acid usage biases of the broad-host range phage, KVP40, were investigated in an attempt to understand the structure and function of its proteins/proteincoding genes, as well as the role of its tRNAs. Synonymous codons in KVP40 were determined to be ATrich at the third codon positions, and their variations are dictated principally by both mutational bias and translational selection. Further analysis revealed that the RSCU of KVP40 is distinct from that of its Vibrio hosts, V. cholerae and V. parahaemolyticus. Interestingly, the expression of the putative highly expressed genes of KVP40 appear to be preferentially influenced by the abundant host tRNA species, whereas the tRNAs expressed by KVP40 may be required for the efficient synthesis of all its proteins in a diverse array of hosts. The data generated in this study also revealed that KVP40 proteins are rich in low molecular weight amino acid residues, and that these variations are influenced primarily by hydropathy, mean molecular weight, aromaticity, and cysteine content. |
| Download PDF | JM45(1)-11.pdf |