| Title |
Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis |
| Author |
Sera Jung1, Semi Park2, Daeha Kim2, and Seung Bum Kim2* |
| Address |
1Graduate School of Public Health and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, 2Department of Biological Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(1),12-15, 2008,
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| DOI |
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| Key Words |
cry1AC, rhizobacteria, Brassica rapa subsp. pekinensis, denaturing gradient gel electrophoresis |
| Abstract |
The effect of genetically modified (GM) Brassica rapa subsp. pekinensis (Chinese cabbage) expressing Bt toxin gene (cry1AC) to the rhizosphere bacterial community was examined using the denaturing gradient gel electrophoresis (DGGE) fingerprinting method. From the visual comparison of the DGGE profiles, there were no significant differences between the profiles of Bt and control rhizosphere in both Suwon and Yesan samples. From the sequence analysis of the individual bands, Sphingomonas sp. of Alphaproteobacteria and several actinobacterial members were identified as the main bacterial taxa in both Suwon and Yesan samples. In the multiple correspondence analysis, no clear separation between Bt and control rhizosphere was seen in both Suwon and Yesan datasets. The profiles of bulk soils were separated from those of rhizosphere. The DGGE fingerprinting analyses indicated that Bt crops did not significantly alter the genetic composition of rhizosphere bacterial communities. |
