| Title |
Phage Types and Pulsed-Field Gel Electrophoresis Patterns of Salmonella enterica serovar Enteritidis Isolated from Humans and Chickens |
| Author |
Sung Hun Kim1, Shukho Kim1, Sung Guen Chun1, Mi-Sun Park1, Jeong Hyun Park2, and Bok-Kwon Lee1* |
| Address |
1Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul 122-701, Republic of Korea, 2Department of Anatomy, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(2),209-213, 2008,
|
| DOI |
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| Key Words |
Salmonella Enteritidis, pulsed-field gel electrophoresis, phage typing, resistance |
| Abstract |
We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken
isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken. |
