Title Lysobacter daecheongensis sp. nov., Isolated from Sediment of Stream Near the Daechung Dam in South Korea
Author Leonid N. Ten1, Hae-Min Jung2, Wan-Taek Im2*, Soon-Ae Yoo1, and Sung-Taik Lee2
Address 1Department of Biology and Medicinal Science, Pai Chai University, Daejeon 302-735, Republic of Korea, 2Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea
Bibliography Journal of Microbiology, 46(5),519-524, 2008,
DOI
Key Words 16S rRNA gene, Xanthomonadaceae, Lysobacter daecheongensis
Abstract A Gram-negative, aerobic, rod shaped, non-spore-forming bacterial strain, designated Dae08T, was isolated from sediment of the stream near Daechung dam in South Korea, and was characterized in order to determine its taxonomic position, using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Dae08T belongs to the family Xanthomonadaceae of the Gammaproteobacteria, and is related to Lysobacter brunescens ATCC 29482T (97.3%). The phylogenetic distances from any other species with validly published names within the genus Lysobacter were greater than 3.7%. The G+C contents of the genomic DNA of strain Dae08T was 69.3 mol%. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with iso-C15:0, iso-C17:1 ω9c, iso-C17:0, iso-C16:0, and iso-C11:0 3-OH as the major acids supported the affiliation of strain Dae08T to the genus Lysobacter. DNA-DNA relatedness between strain Dae08T and its phylogenetically closest neighbour was 28%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Dae08T (= KCTC 12600T) should be classified in the genus Lysobacter as the novel species, for which the name Lysobacter daecheongensis sp. nov. is proposed.