| Title |
Taxonomic Revision of the Nematode-Trapping Fungus Arthrobotrys multisecundaria |
| Author |
Juan Li, Jinkui Yang, Lianming Liang, and Ke-Qin Zhang* |
| Address |
Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P. R. China |
| Bibliography |
Journal of Microbiology, 46(5),513-518, 2008,
|
| DOI |
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| Key Words |
Arthrobotrys multisecundaria, Monacrosporium microscaphoides, serine protease, ITS, β-tubulin,taxonomy |
| Abstract |
The gene encoding an extracellular serine protease was cloned from Arthrobotrys multisecundaria using degenerate primers. The gene was highly similar (99.26%) to protease Mlx from Monacrosporium microscaphoides. To clarify the taxonomic relationship between these species, genes encoding the internal transcribed spacer (ITS) and β-tubulin were also cloned and sequenced from A. multisecundaria and M. microscaphoides, respectively. Homologous analysis of the nuclear (ITS) and protein (β-tubulin) encoding genes showed that the two species of nematode-trapping fungi also shared extensive identity (99.82 and 99.63%, respectively), although they exhibited obvious differences in secondary conidia morphology. Accordingly, a taxonomic revision is recommended, with A. multisecundaria being revised as A. microscaphoides var. multisecundaria. In addition, the identified mutation may better facilitate the study of the sporulation of nematode-trapping fungi. |
