| Title |
Generation of Infectious Transcripts from Korean Strain and Mild Mottle Strain of Potato Virus X |
| Author |
Sun Hee Choi and Ki Hyun Ryu* |
| Address |
Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Seoul 139-774, Republic of Korea |
| Bibliography |
Journal of Microbiology, 46(5),502-507, 2008,
|
| DOI |
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| Key Words |
Potato virus X, in vitro transcription, infectious cDNA clone, plant virus, mild mottle strain, Korean strain |
| Abstract |
Full-length cDNAs of two different strains of Potato virus X (PVX-Kr and PVX-Mo) have been directly amplified by long template reverse transcription polymerase chain reaction (RT-PCR) using the 5’-end primer containing a SP6 or T7 RNA promoter sequence and the virus-specific 3’-end primer, and then constructed in plasmid vectors. Capped in vitro transcripts from cloned full-length cDNAs as well as those RTPCR amplicons proved to be infectious systemically on tobacco plants. Symptom expression on tobacco plants from PVX-Mo transcripts was faster and severer than that from PVX-Kr. In replication stability test of transcripts derived from PVX clones, progeny viruses showed stable replication according to sequencing through passages. This highly infectious transcript system from the full-length cDNA clones for PVX can be useful for recombinant molecules for functional analysis of viral proteins in plant-virus interaction study as well as for expression of foreign protein in planta. |
