| Title |
Overexpression of Bacterioferritin Comigratory Protein (Bcp) Enhances Viability and Reduced Glutathione Level in the Fission Yeast Under Stress |
| Author |
Ga-Young Kang1, Eun-Hee Park2, Kyunghoon Kim1,3, and Chang-Jin Lim1* |
| Address |
1Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2College of Pharmacy, Sookmyung Women’s University, Seoul 140-742, Republic of Korea, 3Korea Basic Science Institute, Chuncheon Center, Chuncheon 200-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 47(1),60-67, 2009,
|
| DOI |
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| Key Words |
fission yeast, bacterioferritin comigratory protein, glutathione, peroxiredoxin, Schizosaccharomyces pombe, stress |
| Abstract |
The structural gene encoding bacterioferritin comigratory protein (Bcp) was amplified using PCR from the genomic DNA of Schizosaccharomyces pombe, and transferred into the shuttle vector pRS316 to generate the recombinant plasmid pBCP10. The bcp+ mRNA level in the pBCP10-containing yeast cells was significantly higher than that in the control yeast cells, indicating that the cloned gene is functioning. The S. pombe cells harboring the plasmid pBCP10 exhibited higher survival on the solid minimal media with hydrogen peroxide, tert-BOOH or cadmium than the control yeast cells. They also exhibited enhanced cellular viability in the liquid media containing the stressful agents. The increased viabilities of the fission yeast cells harboring the plasmid pBCP10 were also obtained with 0.4% glucose or 0.4% sucrose as a sole carbon source, and nitrogen starvation, compared with those of the control yeast cells. The total glutathione (GSH) content and total GSH/GSSG ratio were significantly higher in the yeast cells harboring the plasmid pBCP10 than in the control yeast cells. In brief, the S. pombe Bcp plays a protective role in the defensive response to oxidative stress possibly via up-regulation of total and reduced glutathione levels. |
