| Title |
Induction of Growth Phase-Specific Autolysis in Bacillus subtilis 168 by Growth Inhibitors |
| Author |
Jin-Kyo Chung1, Hyun Ee Yoon2, Ha Chul Shin3, Eun-Young Choi4, and Woo-Hyeon Byeon5* |
| Address |
1Department of Physiology, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Central Lab., Seoul National University Hospital, Seoul 110-744, Republic of Korea, 3FCB-Pharmicell Co., Ltd., Seongnam-si, Gyeonggi-do 462-120, Republic of Korea, 4Department of Molecular Microbiology and Immunology, Life Sciences Center, School of Medicine, University of Missouri-Columbia, Columbia, Missouri 65212, USA, 5Department of Molecular Biosciences, Kangwon National University, Chuncheon 200-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 47(1),50-59, 2009,
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| DOI |
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| Key Words |
autolysis, B. subtilis 168, diauxic point |
| Abstract |
Growth phase-specific autolysis of Bacillus subtilis by inhibitors of membrane permeability, inhibitors of macromolecule biosynthesis, inhibitors of cell wall biosynthesis and detergents were tested and characterized in glucose limited liquid medium. The minimum autolysis induction concentration (MAIC) of test compounds, which was at least 1/20th lower than the conventional autolysis induction concentration, induced autolysis only for cells at the glucose exhaustion point (diauxic point) of the growth phase, while it was not induced for cells at pre- and post-diauxic points. Inhibitors of macromolecule synthesis that are not known for inducing autolysis, such as chloramphenicol, rifampicin, nalidixic acid, and detergents, also induced specific autolysis. Two types of autolysis corresponding to the concentrations of compounds are distinguished: concentration-sensitive and concentration-insensitive types. |
