Title Note] Antibacterial Activity of Recombinant hCAP18/LL37 Protein Secreted from Pichia pastoris
Author Soon-ja Kim1*, Renshu Quan1, Sung-Jin Lee2, Hak-Kyo Lee3, and Joong-Kook Choi1
Address 1Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea, 2Department of Animal Life Resource Science, Kangwon National University, Chuncheon 200-701, Republic of Korea, 3Gyeonggi Regional Research Center, Hankyong National University, Ansung 456-749, Republic of Korea
Bibliography Journal of Microbiology, 47(3),358-362, 2009,
Key Words hCAP18/ LL37, antimicrobial peptide (AMP), antibacterial activity, Pichia pastoris
Abstract Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZαA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.