Title Genotypic Characteristics of Haemophilus influenzae Isolates from Pediatric Pneumonia Patients in Chengdu City, Sichuan, China
Author Tian Guozhong1, Zhang Li2, Li Machao1, Wang Xiaolei2, Zheng Yuhong1, Li Xiaojing2, Huang Cheng2, Li Xuechun2, Xie Yongqiong2, Xu Li1, Ren Hongyu1, and Shao Zhujun1*
Address 1State Key Laboratory for Infectious Diseases Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, P.O. Box 5, Beijing 102206, P. R. China, 2Chengdu Children Hospital, No.137 Taisheng South Road, Chengdu City 610017, P. R. China
Bibliography Journal of Microbiology, 47(4),494-497, 2009,
Key Words Haemophilus influenzae, multilocus sequence typing, biotyping, serotyping
Abstract Two hundred and seventy-three Haemophilus influenzae strains isolated from pediatric pneumonia patients in China were studied. We used Multilocus Sequence Typing (MLST) to analyze genotypic characteristics. All strains were biotyped and serotyped. Relatedness and patterns of genes among isolates were determined by the analysis of MLST and eBURST. H. influenzae primarily causes acute pneumonia in children under 1 year old. Nontypeable H. influenzae was responsible for most cases of pediatric pneumonia. All 273 strains were classified into eight biotypes. They mostly belonged to the I, II, and III biotypes (17.6%, 43.6%, and 22.7%, respectively). 62 strains (22.7%) produced β-lactamase. We found 28 novel alleles. Fifty different STs were found by MLST, of which 39 were novel. These were ST477 through ST508 and ST521 through ST527. Group 17 and predicted founders 503 were new groups in this study. No STs correlated with strains from Korea, which is adjacent to China. The H. influenzae strains from China appeared to have heterogeneous ST types patterns which may be the reason no outbreaks or epidemics of H. influenzae infections have occurred in Chengdu city, Sichuan, China.