| Title |
NOTE] Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Copolyesters with a High Molar Fraction of 3-Hydroxyvalerate by an Insect-Symbiotic Burkholderia sp. IS-01 |
| Author |
Do Young Kim1, Doo-Sang Park2, Soon Bum Kwon3, Moon Gyu Chung3, Kyung Sook Bae2, Ho-Yong Park1, and Young Ha Rhee3* |
| Address |
1Industrial Bio-materials Research Center, KRIBB, Daejeon 305-806, Republic of Korea, 2Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea, 3Department of Microbiology, Chungnam National University, Daejeon 305-764, Republic of Korea |
| Bibliography |
Journal of Microbiology, 47(5),651-656, 2009,
|
| DOI |
|
| Key Words |
Burkholderia, insect-symbiotic microorganism, levulinic acid, polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)], PHA synthase |
| Abstract |
Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB- co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity). |
