| Title |
Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae |
| Author |
Daiwen Xiao1, Yongchang Yang1, Hua Liu1, Hua Yu1, Yingjun Yan1, Wenfang Huang1*, Wei Jiang1, Weijin Liao1, Qi Hu2, and Bo Huang2 |
| Address |
1Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu 610072, P. R. China, 2Laboratory Medicine Department, Chongqing Medical University, Chongqing 400016, P. R. China |
| Bibliography |
Journal of Microbiology, 47(5),646-650, 2009,
|
| DOI |
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| Key Words |
identification, K. pneumoniae, SELDI-TOF MS |
| Abstract |
A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae. |
