Title Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae
Author Daiwen Xiao1, Yongchang Yang1, Hua Liu1, Hua Yu1, Yingjun Yan1, Wenfang Huang1*, Wei Jiang1, Weijin Liao1, Qi Hu2, and Bo Huang2
Address 1Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu 610072, P. R. China, 2Laboratory Medicine Department, Chongqing Medical University, Chongqing 400016, P. R. China
Bibliography Journal of Microbiology, 47(5),646-650, 2009,
DOI
Key Words identification, K. pneumoniae, SELDI-TOF MS
Abstract A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae.