| Title |
Role of a Burkholderia pseudomallei Polyphosphate Kinase in an Oxidative Stress Response, Motilities, and Biofilm Formation |
| Author |
Suda Tunpiboonsak1, Rungrawee Mongkolrob1, Kaniskul Kitudomsub2, Phawatwaristh Thanwatanaying3, Witcha Kiettipirodom4, Yanin Tungboontina4, and Sumalee Tungpradabkul1* |
| Address |
1Department of Biochemistry, Mahidol University, Rama VI Road, Bangkok 10400, Thailand, 2Department of Biology, Mahidol University, Rama VI Road, Bangkok 10400, Thailand, 3Department of Biotechnology Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand, 4Biological Science, International College, Mahidol University, Rama VI Road, Bangkok 10400, Thailand |
| Bibliography |
Journal of Microbiology, 48(1),63-70, 2010,
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| DOI |
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| Key Words |
biofilm, B. mallei, B. pseudomallei, flagellin, oxidative stress, polyphosphate kinase |
| Abstract |
Burkholderia pseudomallei, a motile and rod Gram-negative bacterium, is the causative agent of melioidosis. The bacterium is an intracellular pathogen and that motility is generally crucial for their survival in a natural environment and for systemic infection inside a host. We report here a role of B. pseudomallei polyphosphate kinase in virulence, such as an oxidative stress response, motilities and biofilm formation. The polyphosphate kinase (ppk) mutant is susceptible to hydrogen peroxide in an oxidative stress condition, unable to perform swimming, swarming motilities, and has lower density biofilm forming capacity than the wild-type strain. We also demonstrated that both polyphosphate kinase and motile flagella are essential and independently involved in biofilm formation. The B. pseudomallei flagellin (fliC) mutant and B. mallei, a nonmotile species, are shown to produce higher density biofilm formation than the ppk mutant, but less than wild type B. pseudomallei. |
