| Title |
Potentiation of Bacterial Killing Activity of Zinc Chloride by Pyrrolidine Dithiocarbamate |
| Author |
Eun-Kyoung Choi1, Hye-Hyang Lee1, Mi-Sun Kang1, Byung-Gook Kim2, Hoi-Soon Lim2, Seon-Mi Kim2, and In-Chol Kang1,2* |
| Address |
1Brain Korea 21 Program, Chonnam National University Dental School, Gwangju 500-757, Republic of Korea, 2Dental Science Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(1),40-43, 2010,
|
| DOI |
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| Key Words |
oral malodor, zinc, pyrrolidine dithiocarbamate, bactericidal |
| Abstract |
Zinc has antimicrobial activity and zinc salts including zinc chloride (ZnCl2) have been used for the control of oral malodor. In this study, we hypothesized that pyrrolidine dithiocarbamate (PDTC), a zinc ionophore, may enhance antimicrobial efficacy of ZnCl2. The bactericidal effectiveness of ZnCl2 alone (0.5-8 mM) or in
combination with PDTC (1 or 10 μM) was evaluated by in vitro short (1 h) time-killing assays against Fusobacterium nucleatum and Porphyromonas gingivalis. Only a slight viability decrease was observed with ZnCl2 or PDTC alone after 1-h incubation. By contrast, combination of ZnCl2 and PDTC could achieve a more than 100-fold viability reduction compared with ZnCl2 or PDTC alone in F. nucleatum and P. gingivalis. Therefore, PDTC greatly enhanced the bactericidal activity of ZnCl2 against the oral malodor-producing bacteria. These results suggest that use of PDTC may be useful for enhancing bactericidal activity of antimalodor regimens of zinc salts. |
