| Title |
Isolation and Characterization of Marine Pigmented Bacteria from Norwegian Coastal Waters and Screening for Carotenoids with UVA-Blue Light Absorbing Properties |
| Author |
Marit H. Stafsnes1, Kjell D Josefsen2, Geir Kildahl-Andersen3, Svein Valla1, Trond E. Ellingsen2, and Per Bruheim1,2* |
| Address |
1Department of Biotechnology, Norwegian University of Science and Technology Sem Sælands vei 6/8, NO-7491 Trondheim, Norway, 2SINTEF Materials and Chemistry, Department of Biotechnology, Sem Sælands vei 2a, NO-7465 Trondheim, Norway, 3Department of Chemistry, Norwegian University of Science and Technology Sem Sælands vei 6/8, NO-7491 Trondheim, Norway |
| Bibliography |
Journal of Microbiology, 48(1),16-23, 2010,
|
| DOI |
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| Key Words |
bacterial culture collection, high throughput screening, LC-MS, pigments, carotenoids |
| Abstract |
Microbial culture collections are important resources for isolation of natural compounds with novel properties. In this study, a culture collection of around 1,500 pigmented heterotrophic bacteria was established. The bacteria were isolated from the sea surface microlayer at different sampling sites along the mid-part of the Norwegian coast. The bacterial isolates produced pigments of various coloration (e.g. golden, yellow, red, pink and orange). Methanol extracts of sixteen isolates were characterized with LC-Diodearray-TOF mass spectrometry analysis. The number of pigments per isolate varied considerably, and a tentative identification of the pigments was performed based on UV-absorbance profile and molecular formula
assignation based on the accurate mass determination. The LC-MS analyses evealed that most of the pigments probably were carotenoids. Furthermore, we developed a high throughput LC-MS method for characterization and screening of a larger sub-fraction (300 isolates) of the culture collection. The aim was to screen and identify bacterial isolates producing carotenoids that absorb light in the UVA-Blue light. Six of the bacterial strains were selected for detailed investigation, including 16s rRNA sequencing, preparative HPLC for purification of major carotenoids and subsequent structural elucidation with NMR. Among the identified
carotenoids were zeaxanthin, nostoxanthin and sarcinaxanthin, some with novel glycosylation patterns. |
