| Title |
Genetic Introduction of Foreign Genes to Pleurotus eryngii by Restriction Enzyme-Mediated Integration |
| Author |
Won Noh1, Sang-Woo Kim1, Dong-Won Bae2, Jae-Yean Kim3, and Hyeon-Su Ro1,3* |
| Address |
1Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Chinju 660-701, Republic of Korea, 2Central Instrument Facility, Gyeongsang National University, Chinju 660-701, Republic of Korea, 3Division of Applied Life Science and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Chinju 660-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(2),253-256, 2010,
|
| DOI |
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| Key Words |
hygromycin, P. eryngii, REMI, transformation |
| Abstract |
Pleurotus eryngii was transformed via restriction enzyme-mediated integration. In order to construct the transformation plasmid, the enhanced cyan fluorescent protein (ECFP) gene was ligated next to the gpd promoter of the plasmid pAN7-1. Transformation was facilitated via the heat treatment of a transformation mixture containing 1 μg of the HindIII-digested plasmid DNA and 106 mushroom protoplasts in 40% polyethyleneglycol solution, resulting in 10-40 hygromycin-resistant transformants. Successful transformation was evidenced by PCR, Southern blot, and confocal fluorescence microscopic analyses on the selected transformants. To date, this is the first report on the transformation of P. eryngii by REMI technique. |
