Title Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414
Author Li Wang Wang1, Xiao-Fei Liu2, Shi Yun2, Xiao-Peng Yuan2,3, Xu-Hu Mao2, Chao Wu2, Wei-Jun Zhang2, Kai-Yun Liu2, Gang Guo2, Dong-Shui Lu2, Wen-De Tong2, Ai-Dong Wen1*, and Quan-Ming Zou1*
Address 1Department of Pharmacy, Xijing Hospital, the Fourth Military Medical University, Xi`an 710032, P. R. China, 2Department of Clinical Microbiology and Immunology, School of Medical Laboratory, the Third Military Medical University, Chongqing 400038, P. R. China, 3Department of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, P. R. China
Bibliography Journal of Microbiology, 48(2),223-228, 2010,
Key Words H. pylori, multivalent vaccine, urease B subunit, H. pylori adhesin A, heat shock protein A
Abstract A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.