| Title |
In Vivo Studies with a Candida tropicalis Isolate Exhibiting Paradoxical Growth In Vitro in the Presence of High Concentration of Caspofungin |
| Author |
Sedigh Bayegan1, Laszlo Majoros1*, Gabor Kardos1, Adam Kemény-Beke2, Cecilia Miszti1, Renato Kovacs1, and Rudolf Gesztelyi3 |
| Address |
1Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary, 2Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary, 3Department of Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary |
| Bibliography |
Journal of Microbiology, 48(2),170-173, 2010,
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| DOI |
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| Key Words |
caspofungin, paradoxical growth, murine, C. tropicalis |
| Abstract |
We investigated the activity of caspofungin against a Candida tropicalis clinical isolate showing paradoxical growth in vitro. BALB/c mice immunosuppressed by cyclophosphamide were infected intraperitoneally using 107 CFU/mouse. Caspofungin was administered intraperitoneally once daily for 5 days or as a single dose using the following doses: 0.12, 0.25, 1, 2, 3, 5, and 15 mg/kg. The single dose of caspofungin was effective only at 5 and 15 mg/kg concentrations (100% survival). Five-day caspofungin treatment led to 100% survival at doses of 1 mg/kg or higher. Caspofungin treatment significantly decreased the number of viable yeasts in the peritoneal lavage samples as well as in the infected abscesses at doses 1, 3, 5, and 15 mg/kg caspofungin as compared to the untreated control (P<0.001 in all cases), and even to the group treated with 0.12 mg/kg caspofungin (P<0.05 in all cases). At 2 mg/kg caspofungin dose, sterilization of the internal organs was reproducibly incomplete, suggesting that the role of paradoxical growth in the late clinical failure cannot be excluded. |
