Title Evaluation of Insecticidal Activity of a Bacterial Strain, Serratia sp. EML-SE1 against Diamondback Moth
Author Hyung Uk Jeong1, Hye Yeon Mun1, Hyung Keun Oh1, Seung Bum Kim2, Kwang Yeol Yang1, Iksoo Kim1, and Hyang Burm Lee1*
Address 1College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea, 2Department of Microbiology, Chungnam National University, Daejeon 305-764, Republic of Korea
Bibliography Journal of Microbiology, 48(4),541-545, 2010,
DOI
Key Words diamondback moth (Plutella xylostella), insecticidal activity, Serratia sp., storage period
Abstract To identify novel bioinsecticidal agents, a bacterial strain, Serratia sp. EML-SE1, was isolated from a dead larva of the lepidopteran diamondback moth (Plutella xylostella) collected from a cabbage field in Korea. In this study, the insecticidal activity of liquid cultures in Luria-Bertani broth (LBB) and nutrient broth (NB) of a bacterial strain, Serratia sp. EML-SE1 against thirty 3rd and 4th instar larvae of the diamondback moth was investigated on a Chinese cabbage leaf housed in a round plastic cage (Ø 10×6 cm). 72 h after spraying the cabbage leaf with LBB and NB cultures containing the bacterial strain, the mortalities of the larvae were determined to be 91.7% and 88.3%, respectively. In addition, the insecticidal activity on potted cabbage containing 14 leaves in a growth cage (165×83×124 cm) was found to be similar to that of the plastic cage experiment. The results of this study provided valuable information on the insecticidal activity of the liquid culture of a Serratia species against the diamondback moth.