| Title |
Newly Identified CpG ODNs, M5-30 and M6-395, Stimulate MouseNewly Identified CpG ODNs, M5-30 and M6-395, Stimulate Mouse Immune Cells to Secrete TNF-α and Enhance Th1-Mediated Immunity |
| Author |
Sun-Shim Choi1, Eunkyung Chung2*, and Yu-Jin Jung3* |
| Address |
1Department of Molecular and Medical Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Biowells Inc. and Department of Biomedical Science, Hallym University, Chuncheon 200-702, Republic of Korea, 3Department of Biological Science and Medical and Bio-material Research Center, Kangwon National University, Chuncheon 200-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(4),512-517, 2010,
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| DOI |
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| Key Words |
TLR, CpG ODN, murine immune system, TNF-α, IFN-γ |
| Abstract |
Bacterial CpG motifs are known to induce both innate and adaptive immunity in infected hosts via toll-like receptor 9 (TLR9). Because small oligonucleotides (ODNs) mimicking bacterial CpG motifs are easily synthesized, they have found use as immunomodulatory agents in a number of disease models. We have developed a novel bioinformatics approach to identify effective CpG ODN sequences and evaluate their function as TLR9 ligands in a murine system. Among the CpG ODNs we identified, M5-30 and M6-395 showed significant ability to stimulate TNF-α and IFN-γ production in a mouse macrophage cell line and mouse splenocytes, respectively. We also found that these CpG ODNs activated cells through the canonical NF-κB signaling pathway. Moreover, both CpG ODNs were able to induce Th1-mediated immunity in Mycobacterium tuberculosis (Mtb)-infected mice. Our results demonstrate that M5-30 and M6-395 function as TLR9-specific ligands, making them useful in the study of TLR9 functionality and signaling in mice. |
