| Title |
Inhibitory Effect of the Essential Oil from Chamaecyparis obtusa on the Growth of Food-Borne Pathogens |
| Author |
Mi-Jin Park1, Won-Sil Choi1, Ha-Young Kang1, Ki-Seob Gwak2, Geun-Shik Lee3, Eui-Bae Jeung3, and In-Gyu Choi2,4* |
| Address |
1Division of Green Business Management, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul 130-712, Republic of Korea, 2Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, 3College of Veterinary Medicine, Chungbuk National University, Chungbuk 361-763, Republic of Korea, 4Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(4),496-501, 2010,
|
| DOI |
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| Key Words |
C. obtusa, essential oil, antibacterial activity, terpinen-4-ol |
| Abstract |
In this study, the antibacterial activity of essential oil from Chamaecyparis obtusa (Sieb. et Zucc) leaves and twigs was investigated. The test strains were Klebsiella pneumoniae, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Legionella pneumophila, and Methicilline-resistant Staphylococcus aureus. Antibacterial activity was estimated by measuring bacterial growth inhibition. Histopathological examination was also performed. C. obtusa oil distinctly inhibited the growth of all test strains and exhibited the strongest antibacterial activity against L. monocytogenes. It was chromatographically divided into several fractions. The fractions were further tested against antibacterial activity and their chemical compositions were analyzed. The fraction containing terpinen-4-ol (TA) showed high antibacterial activity toward all strains tested. Tests with authentic samples showed that TA played a major role in the antibacterial activity of C. obtusa oil, and in a mice test, the oil actively minimized inflammation by S. aureus. |
