| Title |
NOTE] Initial Acidic pH Is Critical for Mycelial Cultures and Functional Exopolysaccharide Production of an Edible Mushroom, Laetiporus sulphureus var. miniatus JM 27 |
| Author |
Min Jeong Seo1, Min Jeong Kim2, Hye Hyeon Lee2, Sung Ryeal Kim2, Byoung Won Kang3, Jeong Uck Park3, En Ju Rhu4, Yung Hyun Choi5, and Yong Kee Jeong1,2,3* |
| Address |
1Department of Medical Bioscience, Dong-A University, Busan 604-714, Republic of Korea, 2Department of Biotechnology, Dong-A University, Busan 604-714, Republic of Korea, 3Medi-Farm Industrialization Research Center, Dong-A University, Busan 604-714, Republic of Korea, 4Department of Cosmetology, Hanseo University, Chung Nam 356-706, Republic of Korea, 5Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan 614-050, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(6),881-884, 2010,
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| DOI |
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| Key Words |
mushroom, Laetiporus sulphureus var. miniatus, oxalic acid, p53, U937 |
| Abstract |
We conducted a time course experiment on mycelial cultures of Laetiporus sulphureus var. miniatus. The strain showed significant survival in an initial pH range of 2.0 to 7.0 for 24 days, during which time oxalic acid was accumulated. A structural analysis of purified exopolysaccharide suggested that it contained 96.1% glucose, and the mode of linkage was mainly-4-Glcp-(1-units, with branches at the C-6 position consisting of a Glcp-(1-4) linked side chain. An exopolysaccharide purified from the acidophilic strain was added to cultured U937 cells, resulting in significantly increased transcription levels of p53 and p21 genes. |
