| Title |
NOTE] Analysis of Cytoplasmic Membrane Proteome of Streptococcus pneumoniae by Shotgun Proteomic Approach |
| Author |
Chi-Won Choi1,2, Sung-Ho Yun1, Sang-Oh Kwon1,3, Sun-Hee Leem4, Jong-Soon Choi1,3, Chi-Young Yun2, and Seung Il Kim1* |
| Address |
1Devision of Life Science, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea, 2Department of Biology, Daejeon University, Daejeon 300-716, Republic of Korea, 3Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea, 4Department of Biology and Biomedical Science, Dong-A University, Busan 604-714, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(6),872-876, 2010,
|
| DOI |
|
| Key Words |
Streptococcus pneumoniae, membrane proteome, 1-DE/LC/MS-MS |
| Abstract |
In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for
metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferse systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate
precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae. |
