| Title |
NOTE] Bacillus gaemokensis sp. nov., Isolated from Foreshore Tidal Flat Sediment from the Yellow Sea |
| Author |
Min-Young Jung1,2, Woon Kee Paek3, In-Soon Park1, Jeong-Ran Han1, Yeseul Sin1, Jayoung Paek1, Moon-Soo Rhee1, Hongik Kim4, Hong Seok Song5, and Young-Hyo Chang1* |
| Address |
1Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea, 2Department of Microbiology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea, 3National Science Museum, Daejeon 305-705, Republic of Korea, 4R&D Division, Vitabio Inc., Daejeon 300-824, Republic of Korea, 5R&D Division, Korea Gas Corporation, Ansan 426-790, Republic of Korea |
| Bibliography |
Journal of Microbiology, 48(6),867-871, 2010,
|
| DOI |
|
| Key Words |
B. cereus, B. gaemokensis sp. nov., phylogenetic, new species |
| Abstract |
A Gram-positive, rod-shaped, endospore-forming organism, strain BL3-6T, was isolated from tidal flat sediments of the Yellow Sea in the region of Tae-An. A 16S rRNA gene sequence analysis demonstrated that this isolate belongs to the Bacillus cereus group, and is closely related to Bacillus mycoides (99.0% similarity), Bacillus thuringiensis (99.0%), Bacillus weihenstephanensis (99.0%), Bacillus cereus (98.9%), Bacillus anthracis (98.8%), and Bacillus pseudomycoides (98.1%). The phylogenetic distance from any validly described Bacillus species outside the Bacillus cereus group was less than 95.6%. The DNA G+C content of the strain was 39.4 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C14:0 (17.8%), iso-C16:0 (15.8%), and iso-C12:0 (11.3%). The diagnostic amino acid of the cell wall was mesodiaminopimelic acid and the major cell wall sugar was galactose. The results of DNA-DNA hybridization (<55.6%) and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain BL3-6T from the published Bacillus species. BL3-6T therefore represents a new species, for which the name Bacillus gaemokensis sp. nov. is proposed, with the type strain BL3-6T (=KCTC 13318T =JCM 15801T). |
