| Title |
Endophytic Fungus Trichothecium roseum LZ93 Antagonizing Pathogenic Fungi In Vitro and Its Secondary Metabolites |
| Author |
XiaoMei Zhang1,2, GuoHong Li3, Juan Ma1, Ying Zeng1, WeiGuang Ma2, and PeiJi Zhao1* |
| Address |
1The State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China, 2College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650200, P. R. China, 3Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, P. R. China |
| Bibliography |
Journal of Microbiology, 48(6),784-790, 2010,
|
| DOI |
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| Key Words |
antifungal, antagonism, endophytes, pathogenic fungi, trichothecin |
| Abstract |
The endophytic fungus Trichothecium roseum LZ93 from Maytenus hookeri was found to antagonize other pathogenic fungi in vitro. To identify which compound contributed substantially to the antagonism, we fermented the strain and purified its fermentation products. Eleven compounds were obtained, including two
trichothecenes, five rosenonolactones, two cardiotonic cyclodepsipeptides, and two sterols. Compound 11β-hydroxyrosenonolactone (1) was assigned according to 1D and 2D-NMR data for the first time. At the same time, the 1H and 13C-NMR assignments for 6β-hydroxyrosenonolactone (2) were revised. Of all of them, only
trichothecin (6) showed strong antifungal activity. Based on our observations of the antagonistic activity and the other experimental results, we suggest that the antifungal compound trichothecin was the main contributor to the antagonistic action of T. roseum LZ93. |
